Level of receptor complex molecule expression of human blood T-lymphocytes under conditions of their UV-irradiation

• Published in: Radiat͡s︡ionnai͡a︡ biologii͡a︡, radioėkologii͡a Vol. 51; no. 2; p. 258
• Main Authors: Artiukhov, V G, Putintseva, O V, Vdovina, V A, Koltakov, I A, Pashkov, M V, Vasilenko, D V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-03-2011
• Subjects: CD3 Complex - biosynthesis; CD4 Antigens - biosynthesis; CD8 Antigens - biosynthesis; Cell Membrane - immunology; Cell Membrane - metabolism; Cell Membrane - radiation effects; Cells, Cultured; Dose-Response Relationship, Radiation; Flow Cytometry; Humans; Immunoenzyme Techniques; Receptors, Antigen, T-Cell - biosynthesis; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; T-Lymphocytes - radiation effects; Ultraviolet Rays
• ISSN: 0869-8031

The influence of a wide range of doses of UV-light (240-390 nm) on the expression level of receptor complex molecules (CD3, CD4, CD8 markers) on the membrane surface of human blood T-lymphocytes has been studied using flow cytometry and enzyme linked immunosorbent assay. UV-light at small and medium doses (15 1, 453 and 906 J/m2) has been established to have a unidirectional (activating) effect on the expression level of receptor complex molecules, and at a high dose of 1359 J/m2 it can either increase (CD4 and CD8 markers) or reduce (CD3 complexes) the quantity of the analyzed molecules on the membrane surface of T-lymphocytes.