Expression of the starfish complement component C3 gene homologue under the influence of bacterial lipopolysaccharide

The fragment of a homologue of complement component C3 gene has been cloned and sequenced from the starfish, Asterias rubens. Phylogenetic analysis of ArC3-like gene demonstrates that ArC3-like gene has close similarity to C3 gene homologues of Deuterostomia invertebrate animals. High level of ArC3-...

Full description

Saved in:

Bibliographic Details
Published in:	Molekuliarnaia biologiia Vol. 44; no. 1; p. 74
Main Authors:	Mogilenko, D A, Kudriavtsev, I V, Orlov, S V, Kharazova, A D, Polevshchikov, A V
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-01-2010
Subjects:	Animals Asterias - genetics Asterias - immunology Cloning, Molecular Complement C3 - classification Complement C3 - genetics DNA, Complementary - genetics Evolution, Molecular Female Gene Expression Regulation - immunology Lipopolysaccharides - immunology Male Phylogeny
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The fragment of a homologue of complement component C3 gene has been cloned and sequenced from the starfish, Asterias rubens. Phylogenetic analysis of ArC3-like gene demonstrates that ArC3-like gene has close similarity to C3 gene homologues of Deuterostomia invertebrate animals. High level of ArC3-like gene expression was identified in circulating cells (coelomocytes), in a gut's derivate (hepatopancreas) and in male gonada but not in stomach, female gonad and rectal gland of A. rubens starfish. ArC3-like gene expression was shown in all types of starfish coelomocytes: in lymphocyte-like cells, granular and nongranular amebocytes. Injection of bacterial lipopolysaccharide (LPS) solution into the coelomic cavity of starfish leads to the increase of ArC3-like gene expression in coelomocytes and hepatopancreas over the control level of sterile sea water injection. The level of ArC3-like gene expression increased in response to LPS reaching the maximum 6 h after the stimulation, and decreased to basal level 24 h after the stimulation. Injection of LPS solution stimulated the increase of ArC3-like gene expression level in hepatopancreas reaching the maximum 6-12 h after the stimulation, and the level of mRNA of ArC3-like gene had still been increased 48 h after LPS injection. The data demonstrates sustained positive regulation of ArC3-like gene expression under the influence of LPS.
ISSN:	0026-8984