Induction of gonadal intersex in genotypic male rainbow trout (Oncorhynchus mykiss) embryos following immersion in estradiol-17beta

• Published in: Molecular reproduction and development Vol. 56; no. 4; pp. 495 - 501
• Main Authors: Krisfalusi, M, Nagler, J J
• Format: Journal Article
• Language: English
• Published: United States 01-08-2000
• Subjects: Animals; Disorders of Sex Development; Embryo, Nonmammalian - drug effects; Embryo, Nonmammalian - physiology; Estradiol - pharmacology; Female; Male; Microscopy, Polarization; Oncorhynchus mykiss - embryology; Ovary - drug effects; Ovary - metabolism; Receptors, Estrogen - genetics; Receptors, Estrogen - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Sex Determination Analysis; Sex Differentiation; Testis - drug effects; Testis - embryology; Testis - metabolism; Time Factors
• ISSN: 1040-452X

This investigation was conducted to determine the initial period of gonadal sensitivity to estrogen in genetically male rainbow trout (Oncorhynchus mykiss). Fish were immersed in approximately 250 microg estradiol-17beta, (E2)/l for two 2 hr periods during different stages of embryonic development beginning 30 days postfertilization (DPF) and continuing until 68 DPF. Histological analysis of gonad samples indicated a significant proportion of E2-treated fish had intersex gonads; these gonads were primarily comprised of testicular tissue with one or more oocytes scattered throughout. The most sensitive period for altering normal testicular development was found to occur between 44 and 51 DPF (63% intersex), while the labile period in general was determined to span 24 days (from 30 to 54 DPF). Additionally, quantitative RT-PCR was used to measure estrogen receptor (ER) mRNA expression in whole individual untreated embryos at six weekly time points throughout the period of E2-exposure. Although the intersex condition was not observed throughout the entire E2-exposure period, ER mRNA was detected at each time point assayed.