Evaluation of an ELISA kit made in our laboratory for intratypic discrimination of poliovirus type 1 strains isolated from acute flask paralysis

• Published in: Mikrobiyoloji bülteni Vol. 36; no. 1; pp. 71 - 77
• Main Authors: Ozkaya, Etem, Arita, Mineo
• Format: Journal Article
• Language: Turkish
• Published: Turkey 01-01-2002
• Subjects: Acute Disease; Antibodies, Monoclonal; Enzyme-Linked Immunosorbent Assay - standards; Feces - virology; Humans; Paralysis - virology; Poliomyelitis - virology; Poliovirus - classification; Poliovirus - immunology; Poliovirus - isolation & purification; Reference Standards; Sensitivity and Specificity
• ISSN: 0374-9096

In this study, we aimed to develop and evaluate an enzyme based immunological method (ELISA) for the intratypic differentiation (whether if Sabin like-SL, or non Sabin like/wild type-NSL) of poliovirus type 1 isolates, in the frame of Polio Eradication Programme (PEP) in Turkey. Five poliovirus type 1 strains which were formerly isolated and identified in our laboratory from fecal samples of acute flask paralysis (AFP) patients, and standard reference strains of vaccine type 1 and prototype wild (Mahoney) polioviruses have been tested into plate wells that bounded with 5 different monoclonal antibodies (mAbs B18, S2-36, M18, B82, M49). The wells which gave high absorbance than cutoff value estimated by comparing negative control wells, were accepted as positive. As a result, the standard poliovirus strains were differentiated correctly, whereas 2 patient isolates were identified as SL and 3 were NSL. In conclusion, our home-made ELISA method found to be successful for intratypic differentiation of poliovirus type 1 isolates, however more detailed studies are necessary in order to increase the susceptibility and specificity of this test.