Detection of antigen-antibody interaction of human adenovirus by the method of surface plasmon resonance

A possibility to detect adenoviral protein--hexon, using specific antibodies by surface plasmon resonance (SPR) was demonstrated. The hexon of the human adenovirus 2 (Ad2) binds to antibodies immobilized on the sensor surface treated by KNCS and protein A Staphylococcus aureus. The specificity of an...

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Bibliographic Details
Published in:	Mikrobiolohichnyĭ zhurnal (Kiev, Ukraine : 1993) Vol. 67; no. 4; p. 58
Main Authors:	Nosach, L M, Boltovets', P M, Povnytsia, O Iu, Zhovnovata, V L, Zakharenko, O M, Snopok, B A, Shyrshov, Iu M, Diachenko, N S
Format:	Journal Article
Language:	Ukrainian
Published:	Ukraine 01-07-2005
Subjects:	Adenoviruses, Human - immunology Adenoviruses, Human - isolation & purification Antibodies, Viral - immunology Antigen-Antibody Complex - analysis Antigen-Antibody Complex - immunology Antigens, Viral - immunology Capsid Proteins - immunology Humans Sensitivity and Specificity Surface Plasmon Resonance
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Summary:	A possibility to detect adenoviral protein--hexon, using specific antibodies by surface plasmon resonance (SPR) was demonstrated. The hexon of the human adenovirus 2 (Ad2) binds to antibodies immobilized on the sensor surface treated by KNCS and protein A Staphylococcus aureus. The specificity of antihexon antibodies was demonstrated by indirect method of fluorescent antibodies (MFA) and cellular variant of the immunoassay (cELISA).
ISSN:	1028-0987