ELISA and RT-PCR-based research of viruses in the ticks collected in the foci of Crimean-Congo fever in Kazakhstan and Tajikistan in 2001-2002

• Published in: Voprosy virusologiĭ Vol. 50; no. 1; p. 23
• Main Authors: Onishchenko, G G, Tumanova, I Iu, Vyshemirskiĭ, O I, Kuhn, J, Seregin, S V, Tiunnikov, G I, Petrova, I D, Tishkova, F kh, Ospanov, K S, Kazakov, S V, Karimov, S K, Esmagambetova, A S, Netesov, S V, Petrov, V S
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-01-2005
• Subjects: Animals; Antigens, Viral - analysis; Arachnid Vectors - classification; Arachnid Vectors - virology; Ecosystem; Enzyme-Linked Immunosorbent Assay; Hemorrhagic Fever Virus, Crimean-Congo - genetics; Hemorrhagic Fever Virus, Crimean-Congo - immunology; Hemorrhagic Fever Virus, Crimean-Congo - isolation & purification; Hemorrhagic Fever, Crimean - epidemiology; Humans; Ixodidae - classification; Ixodidae - virology; Kazakhstan; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral - analysis; Seasons; Species Specificity; Tajikistan; Kazakhstan; Tajikistan
• ISSN: 0507-4088

Different species of ticks were found, in the territories of Kazakhstan and Tajikistan, to be infected with the virus of Crimean-Congo hemorrhagic fever (CKHF). The virologic evaluation included determination of antigen and RNA of the CKHF virus by ELISA and RT-PCR, respectively. The below tick species were found to be involved in the epidemic process: Hyalomma asiaticum, Dermacentor niveus (Kazakhastan) and Hyalomma anatolicum (Tajikistan). The results testify to the fact that Hyalomma ticks are the main carrier of the above virus in the Middle Asia. At the same time, Dermacentor niveus ticks are infection carriers in Kazakhstan.