Sensitivity of PCR method for detection of mycobacteria tuberculosis in patients with lung tuberculosis

• Published in: Georgian medical news no. 118; p. 14
• Main Authors: Dochviri, T Z, Salakaia, A I, Katsitadze, V A, Chigogidze, T G
• Format: Journal Article
• Language: Russian
• Published: Georgia (Republic) 01-01-2005
• Subjects: Adult; Aged; Algorithms; Female; Humans; Male; Middle Aged; Mycobacterium tuberculosis - isolation & purification; Polymerase Chain Reaction; Risk Factors; Tuberculosis, Pulmonary - diagnosis; Tuberculosis, Pulmonary - microbiology; Tuberculosis, Urogenital - diagnosis; Tuberculosis, Urogenital - microbiology; Tuberculosis, Urogenital - urine; Urine - microbiology
• ISSN: 1512-0112

Both hospitalized patients and outpatient clinic patients at the National Centre of Tuberculosis and Lung Diseases of Georgia have been investigated. The group of patients with the tuberculosis of urogenital system, has been studied (newly detected cases), 70 cases in total. The examination of the urine was carried out by the Polymerase Chain Reaction (PCR) method in order to detect Kochi bacillus and by three-time bacterioscopy of urine on acid resistant bacterium. Mycobacterium tuberculosis in urine has been detected in 57 (81,43%) patients by PCR method, and by urine bacterioscopy acid fast bacilli (AFB)(+) in 36 (51,43%) patients. 50 hospitalized patients were examined as a separate group. They had the tuberculosis of lungs and insignificant pathological changes in urogenital system. Among them there was active bacillus secretion in 45 cases by phlegm bacterioscopy AFB(+). Out of 50 patients the mycobacterium tuberculosis in urine was detected in 30 (60%) cases by PCR method. It should be mentioned that according to the urine two-time bacterioscopy, carried out on 50 patients, has not been detected AFB(-) bacillus secretion. It may be concluded that the PCR method is advantageous in detecting of Kochi bacillus in urine. Introduction of this method in medical practice will give us the possibility to determine the risk-group for development of tubercular changes in urinary system associated with lung tuberculosis, in order to control such patients and to carry out the adequate urological examinations.