Nuclei and nucleolar organizing regions in chromosomes of lymphocytes on different stages of periodic disease

• Published in: T͡S︡itologii͡a Vol. 46; no. 4; p. 376
• Main Authors: Karalova, E M, Abroian, L O, Akopian, L O, Karagezian, K G, Magakian, Iu A
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2004
• Subjects: Amyloidosis - blood; Amyloidosis - complications; Amyloidosis - diagnosis; Cell Nucleus - pathology; Cell Nucleus - ultrastructure; Familial Mediterranean Fever - blood; Familial Mediterranean Fever - complications; Familial Mediterranean Fever - diagnosis; Humans; Laser Scanning Cytometry; Lymphocytes - pathology; Lymphocytes - ultrastructure; Nucleolus Organizer Region - pathology; Nucleolus Organizer Region - ultrastructure
• ISSN: 0041-3771

By scanning cytomorphometry a cytological study was first performed on the behavior of nuclei and nucleolar organizing regions (NOR) in chromosomes of peripheral blood lymphocytes of healthy men and of patients with periodic disease (familial Mediterranean fever, FMF) on different stages of development, including its complication with amyloidosis. The volume and total surface of nuclei, the sum total volume and sum total surface of NOR, the mean number of NOR for one nucleus and distribution of nuclei according to NOR number were measured. It is shown that the parameters of nuclei and NOR for patients with FMF on all stages clearly and trustworthy differ from those for healthy men. They are sufficiently informative, can be successfully used in clinical practice and even serve as an early diagnostic test for amyloidosis complication.