Monoclonal antibodies to Mycoplasma pneumoniae antigens

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii no. 2; p. 81
• Main Authors: Gorina, L G, Drobyshevskaia, E I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-03-1999
• Subjects: Animals; Antibodies, Monoclonal - analysis; Antibodies, Monoclonal - isolation & purification; Antibody Specificity; Antigen-Antibody Reactions - immunology; Antigens, Bacterial - immunology; Epitopes - immunology; Fluorescent Antibody Technique; Hemagglutination Tests; Hybridomas - immunology; Immunization; Immunoblotting; Mice; Mice, Inbred BALB C; Molecular Weight; Mycoplasma pneumoniae - immunology
• ISSN: 0372-9311

Approaches to obtaining stable mouse hybridomas, capable of producing monoclonal antibodies (McAb) to M. pneumoniae key antigens, were developed. As the result of hybridization experiments, 7 clones were obtained; of these, 4 clones stably synthesized IgG McAb. Clones H1/H9 and H9/B2 synthesized antibodies to thermolabile, proteinase-sensitive K protein, produced by cytoplasmic membranes of M. pneumoniae cells. The molecular weight of this protein was found to be 90 kD. McAb of clone H1/H9, labeled with horse-radish peroxidase and fluorescein isothiocyanate, specifically reacted with M. pneumoniae antigens in the immunofluorescence test and the enzyme immunoassay (EIA). The sensitivity of EIA was 0.25 ng/ml of antigen protein. These data are prerequisites for the development of diagnostic test systems for the detection of M. pneumoniae antigens in different biological substances obtained from patients with respiratory pathology.