Treatment with prostaglandin F2alpha increases expression of prostaglandin synthase-2 in the rat corpus luteum

• Published in: Prostaglandins & other lipid mediators Vol. 70; no. 1-2; pp. 145 - 160
• Main Authors: Narayansingh, R M, Senchyna, M, Carlson, J C
• Format: Journal Article
• Language: English
• Published: United States 01-09-2002
• Subjects: Animals; Corpus Luteum - drug effects; Corpus Luteum - enzymology; Corpus Luteum - physiology; Dinoprost - pharmacology; Female; Gene Expression; Luteolysis - drug effects; Progesterone - blood; Prostaglandin-Endoperoxide Synthases - biosynthesis; Prostaglandin-Endoperoxide Synthases - genetics; Rats; Reverse Transcriptase Polymerase Chain Reaction; Superovulation - blood
• ISSN: 1098-8823

Recent studies indicate that the corpus luteum (CL) may be a source of prostaglandin F2alpha (PGF2alpha) for regression. We investigated expression of mRNA and protein for prostaglandin G/H synthase (PGHS) in the CL of immature superovulated rats following administration of PGF2alpha. We observed an increase in mRNA for PGHS-2, the induced isoform, at 1 h and protein at 8 and 24 h after treatment. One hour after PGF2alpha, there was also a progressive decrease in plasma progesterone concentration. There were no changes, however, in expression of PGHS-1, the constitutive isoform, over the 24 h sampling period. These results indicate that PGHS-2 increases following PGF2alpha treatment and that expression of this enzyme in the rat CL may contribute to the luteolytic mechanism.