Study of the diagnostic potential of various regions of tick-borne encephalitis virus glycoprotien E

• Published in: Voprosy virusologiĭ Vol. 53; no. 1; p. 36
• Main Authors: Obriadina, A P, Zagriadskaia, Iu E, Savel'eva, N V, Pimenov, V K, Puzyrev, V F, Burkov, A N, Ulanova, T I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-01-2008
• Subjects: Antibodies, Viral - blood; Antibody Specificity; Antigens, Viral - biosynthesis; Antigens, Viral - genetics; Antigens, Viral - immunology; Antigens, Viral - isolation & purification; Chromatography; Computational Biology; Encephalitis, Tick-Borne - blood; Encephalitis, Tick-Borne - diagnosis; Escherichia coli - metabolism; Fluorescent Antibody Technique, Indirect - methods; Genes, Viral - genetics; Humans; Polymerase Chain Reaction; Recombinant Proteins - biosynthesis; Recombinant Proteins - isolation & purification; Viral Envelope Proteins - biosynthesis; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Viral Envelope Proteins - isolation & purification
• ISSN: 0507-4088

Bioinformation techniques were used to analyze envelope glycoprotein of tick-borne encephalitis virus in order to determine the potential diagnostically significant antigenic clusters. Five selected regions of the amino acid sequence of protein E were retranslated to nucleic sequences, by employing the optimum code for E. coli. The resultant DNA fragments were synthesized by polymerase chain reaction from synthetic oligonucleotides and expressed in E. coli cells. The recombinant antigen replicating the region from 296 to 414 aminoacids demonstrated the most significant antigenic activity. The findings lead to the conclusion that this recombinant protein may be considered as a candidate for the development of a diagnostic assay.