Combined enzymatic modification of stevioside and rebaudioside A

Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside and rebaudiosides A with the use of starch as a donor. CGTases produced by B. stearothermophilus B-5076 B. macerans BIO-4m we...

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Bibliographic Details
Published in:	Prikladnaja biohimija i mikrobiologija Vol. 42; no. 1; p. 37
Main Authors:	Kochikian, V T, Markosian, A A, Abelian, L A, Balaian, A M, Abelian, V A
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-01-2006
Subjects:	Bacillus - enzymology Chromatography, Liquid Diterpenes, Kaurane - chemistry Glucosides - chemistry Glucosyltransferases - chemistry Glycosylation Starch - chemistry Stevia - chemistry Sweetening Agents - chemistry
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Summary:	Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside and rebaudiosides A with the use of starch as a donor. CGTases produced by B. stearothermophilus B-5076 B. macerans BIO-4m were the most effective biocatalysts. This method can be successfully used for direct transglycosylation of stevia extract without purification of its individual components.
ISSN:	0555-1099