Theoretical prediction of the antigenic epitopes of severe acute respiratory syndrome (SARS-CoV) proteins and evaluation of their diagnostic value

• Published in: Voprosy virusologiĭ Vol. 50; no. 5; p. 22
• Main Authors: Ulanova, T I, Puzyrev, V F, Riabinina, S A, Burkov, A N, Obriadina, A P
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-09-2005
• Subjects: Amino Acid Sequence; Antigens, Viral - biosynthesis; Antigens, Viral - genetics; DNA Primers; Epitopes - genetics; Escherichia coli - genetics; Escherichia coli - metabolism; Genetic Vectors; Humans; Immunoenzyme Techniques - methods; Membrane Glycoproteins - biosynthesis; Membrane Glycoproteins - genetics; Molecular Sequence Data; Nucleocapsid Proteins - biosynthesis; Nucleocapsid Proteins - genetics; Recombinant Proteins - biosynthesis; SARS Virus - genetics; SARS Virus - immunology; Sequence Analysis; Severe Acute Respiratory Syndrome - diagnosis; Spike Glycoprotein, Coronavirus; Transformation, Bacterial; Viral Envelope Proteins - biosynthesis; Viral Envelope Proteins - genetics; Viral Matrix Proteins - biosynthesis; Viral Matrix Proteins - genetics
• ISSN: 0507-4088

Analysis of the sequence of a number of proteins of the virus causing severe acute respiratory syndrome (SARS-CoV) was used to theoretically predict antigenic epitopes. Nine sequences, encoding for the predicted epitopes in spike, nucleocapside, and membranous SARS CoV proteins, were synthesized by polymyrase chain reaction and cloned into the pGEX 4-T2 vector. The resultant plasmids were expressed in the E. coil cells and purified by glutathione-Sepharose affinity chromatography.