Effect of a corrinoid on Methanosarcina barkeri DNA synthesis

Methanosarcina barkeri is capable of synthesizing large amounts of corrinoids, compounds of the vitamin B12 group, although not cobalamin. In the present work, exogenous cobalamin was demonstrated to upregulate DNA synthesis in M. harkeri cell suspensions incubated under air. The effect is similar t...

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Bibliographic Details
Published in:	Mikrobiologija (Moskva. 1932) Vol. 78; no. 1; p. 5
Main Authors:	Ryzhkova, E P, Briukhanov, A L
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-01-2009
Subjects:	Aerobiosis Anaerobiosis DNA Replication - drug effects DNA, Archaeal - biosynthesis DNA, Archaeal - genetics Methanosarcina barkeri - drug effects Methanosarcina barkeri - genetics Methanosarcina barkeri - metabolism Propionibacterium - growth & development Propionibacterium - metabolism Vitamin B 12 - metabolism Vitamin B 12 - pharmacology
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Summary:	Methanosarcina barkeri is capable of synthesizing large amounts of corrinoids, compounds of the vitamin B12 group, although not cobalamin. In the present work, exogenous cobalamin was demonstrated to upregulate DNA synthesis in M. harkeri cell suspensions incubated under air. The effect is similar to the one in Propionibacterium freudenreichii cells, though less pronounced. The growth of the archaeon under anaerobic conditions was shown to be suppressed by cobalamin and 5,6-dimethylbenzimidazole. The data obtained suggest the presence of a corrinoid-dependent ribonucleotide reductase in the archaeon cells which provides for deoxyribose precursors for DNA biosynthesis independently of the presence of molecular oxygen in the medium. Growth suppression under anoxic conditions by cobalamin and 5,6-dimethylbenzimidazole may be due to a decrease in the concentration of factor III, a polyfunctional corrinoid dominating in M. barkeri cells.
ISSN:	0026-3656