Participation of bone-marrow stem cells in the differentiation of mdx mice striated muscle

• Published in: T͡S︡itologii͡a Vol. 48; no. 5; p. 410
• Main Authors: Mikhaĭlov, V M, Evtifeeva, E V, Serikov, V B, Perverzev, A E, Karmanova, A V, Zenin, V V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2006
• Subjects: Animals; Bone Marrow Transplantation; Cell Differentiation; Chimera; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Injections, Intramuscular; Mice; Mice, Inbred C57BL; Mice, Inbred mdx; Mice, Transgenic; Muscle Fibers, Skeletal - cytology; Muscle, Skeletal - cytology; Stem Cells - physiology
• ISSN: 0041-3771

Two sets of experiments were carried out. The first one involved chimeric mice, obtained by intravenously injections of bone marrow derived cells taken from transgenic C57BL/6 mice, expressing GFP, to 5 Gy X-ray irradiated mdx or C57BL/6 mice. In 2 months M. quadriceps femoris of chimeric mice were destroyed by surgical clamp. Following the next 4-5 weeks, the same muscles were studied for the presence of GFP-positive striated muscle fibres. In the case of chimeric C57BL/6 mice GFP-positive striated muscle fibres were observed in 0.3 +/- 0.5 and in 0.2 +/- 0.3 % of destroyed muscle, and in lateral (control) muscle, consequently. In the case of chimeric mdx mice, positive results were observed in 1.7 +/- 0.4 and in 0.5 +/- 0.3 % of destroyed and control muscles, respectively. In the second set of experiments, the GFP-positive bone marrow cells were used for multiple intramuscular injections to M. quadriceps femoris of C57BL/6 or mdx mice in a dose of 2 x 10(5)-5 x 10(5) cells per mouse. Before injection, GFP-positive bone marrow cells were fractionated in a 63 % Percoll solution and then were exhausted from differentiated cells by magnetic manner using CD4, CD8, CD38, CD45R, CD119, Ly-6G, and F4/80 antibodies. After 2-3 weeks, as many as 0.15 +/- 0.40 and 0.1 +/- 0.2 % of GFP-positive muscle fibres were found in injected and control muscles of C57BL/6 mice, respectively. In the case of mdx mice, the frequency of GFP-positive striated muscle fibres was 2.0 +/- 0.8 and 1.2 +/- 0.6 % for injected and control muscles, respectively. A conclusion is made that bone marrow stem cells can take part in differentiation of mdx mouse muscles after their delivery by needle injections.