Effect of homologous peptides of differentiation factors HLDF and PEDF on preimplantation development of mice in vitro

• Published in: Ontogenez Vol. 33; no. 3; p. 195
• Main Authors: Sakharova, N Iu, Kostanian, I A, Lepikhova, T N, Lepikhov, K A, Malashenko, A M, Navolotskaia, E V, Tombran-Tink, J, Lipkin, V M
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-05-2002
• Subjects: Animals; Blastocyst - drug effects; Cell Differentiation; Cells, Cultured; Embryonic and Fetal Development - drug effects; Embryonic Development - drug effects; Eye Proteins; Female; Mice; Mice, Inbred C57BL; Neoplasm Proteins - chemistry; Nerve Growth Factors; Peptide Fragments - chemistry; Peptide Fragments - pharmacology; Pregnancy; Proteins - chemistry; Proteins - pharmacology; Serpins - chemistry; Serpins - pharmacology
• ISSN: 0475-1450

We studied the effect of synthetic peptides PEDF-6 and HLDF-6 on preimplantation development of mouse embryos in vitro. PEDF-6 peptide corresponds to fragment 351-356 and of pigment epithelium-derived differentiation factor (PEDF), while HLDF-6 peptide corresponds to fragment 84-89 of differentiation factor HLDF isolated from HL-60 cell line. Despite high homology, these peptides had different effects on the early development. PEDF-6 had no effect on the cleavage of 2-4-cell embryos but decelerated blastocyst formation from such embryos and disturbed their structure. In the presence of HLDF-6 the blastomeres divided more actively as compared to the control and a higher number of embryos developed to the blastocyst stage. The effects of both peptides were stage-specific: the affect the embryos at early cleavage stages and, apparently, determine their further development at that moment although do not directly affect formation of the blastocysts.