New ways to increasing the yield of laccase from Panus tigrinus fungi

We optimized the conditions for production of laccase by lignolytic fungi Panus tigrinus 8/18. 2,4-Dimethylphenol was used as an aromatic inductor. The addition of 2,4-dimethylphenol and 2 mM CuSO4 to a rich medium was followed by a tenfold increase in the yield of this enzyme. Additional treatment...

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Bibliographic Details
Published in:	Prikladnaja biohimija i mikrobiologija Vol. 41; no. 5; p. 578
Main Authors:	Chernykh, S M, Leont'evskiĭ, A A, Golovleva, L A
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-09-2005
Subjects:	Basidiomycota - growth & development Basidiomycota - metabolism Caprolactam - analogs & derivatives Cells, Immobilized Copper Sulfate Culture Media Fluorocarbons Laccase - biosynthesis Polymers Xylenes
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Summary:	We optimized the conditions for production of laccase by lignolytic fungi Panus tigrinus 8/18. 2,4-Dimethylphenol was used as an aromatic inductor. The addition of 2,4-dimethylphenol and 2 mM CuSO4 to a rich medium was followed by a tenfold increase in the yield of this enzyme. Additional treatment of the medium with perftoran (oxygen-carrying agent) and immobilization of the fungus on polycaproamide fibers increased significantly laccase activity in the medium. The conditions for cultivation of P. tigrinus fungi were optimized. It allowed us to increase laccase activity in the medium by 25 times (compared to activity of the enzyme obtained with previously described methods).
ISSN:	0555-1099