Use of PCR methods for identification of Listeria monocytogenes in milk

The aim of this work was to estimate the limit of Listeria monocytogenes cfu in polymerase chain reaction (PCR) for a DNA fragment of listeriolysine O (hly A) gene. The PCR method, with used primers selected in areas of the listeriolysin O gene, allows to differentiate L. monocytogenes strains from...

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Bibliographic Details
Published in:	Medycyna doświadczalna i mikrobiologia Vol. 50; no. 3-4; p. 251
Main Authors:	Wieckowska, M, Kotłowski, R, Kur, J, Rudnicka, W
Format:	Journal Article
Language:	Polish
Published:	Poland 1998
Subjects:	Animals DNA, Bacterial - analysis Listeria monocytogenes - isolation & purification Milk - microbiology Polymerase Chain Reaction - methods Species Specificity
Online Access:	Get more information
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Summary:	The aim of this work was to estimate the limit of Listeria monocytogenes cfu in polymerase chain reaction (PCR) for a DNA fragment of listeriolysine O (hly A) gene. The PCR method, with used primers selected in areas of the listeriolysin O gene, allows to differentiate L. monocytogenes strains from other Listeria species. The amplified fragment (456 bp) of hly A gene was obtained for all strains L. monocytogenes and no other Listeria species. The PCR method with the selected primers allowed to detect 50-500 cfu L. monocytogenes/ml suspended in water or milk. Among 20 samples of raw milk from cows, 10 samples contained > 50 cfu L. monocytogenes/ml. Obtained results indicate that the PCR assay of L. monocytogenes identification is technically simple and may be conduct with minimal time. So, it could be recommended as quick diagnostic method in identification L. monocytogenes in milk.
ISSN:	0025-8601