Isolation of strains of the virus of hemorrhagic fever with renal syndrome in cell culture

Four strains of hemorrhagic fever with renal syndrome (HFRS) virus were isolated from the lungs of Cl. glareolus caught in natural foci of HFRS in the European USSR. Preliminary experiments using enzyme-immunoassay (EIA) established the appurtenance of HFRS antigen in lung suspensions to the "w...

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Bibliographic Details
Published in:	Voprosy virusologiĭ Vol. 29; no. 4; p. 497
Main Authors:	Bashkirtsev, V N, Tkachenko, E A, Dzagurova, T K, Ryl'tseva, E V
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-07-1984
Subjects:	Animals Antibodies, Viral - analysis Arvicolinae - microbiology Bashkiria Cell Line Cercopithecus aethiops Fluorescent Antibody Technique Hantavirus - immunology Hantavirus - isolation & purification Humans Immunosorbent Techniques Kidney Lung - microbiology Rats Rats, Inbred Strains RNA Viruses - isolation & purification Virus Cultivation Bashkiria
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Summary:	Four strains of hemorrhagic fever with renal syndrome (HFRS) virus were isolated from the lungs of Cl. glareolus caught in natural foci of HFRS in the European USSR. Preliminary experiments using enzyme-immunoassay (EIA) established the appurtenance of HFRS antigen in lung suspensions to the "western" serotype. Four strains were isolated in Vero-E6 cell culture after several blind passages and identified as the "western" serotype of HFRS virus. One stram originally isolated in Wistar rats and passaged in them 4 times and then adapted to Vero-E6 cells showed a closer antigenic relationship with the "eastern" HFRS virus serotype.
ISSN:	0507-4088