Analysis of protected peptides--ACTH(1-24) fragments using high performance liquid chromatography

The applicability of reversed-phase ion-pair HPLC for separating the ACTH-(1-24) fragments obtained at the final stage of the synthesis has been examined. Optimization of chromatographic conditions (selection of proper packing material, eluent and concentration of ion-pairing reagent) for separation...

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Bibliographic Details
Published in:	Bioorganicheskaia khimiia Vol. 10; no. 11; p. 1448
Main Authors:	Titova, E V, Chlenov, M A, Kudriashov, L I
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-11-1984
Subjects:	Adrenocorticotropic Hormone - analogs & derivatives Amino Acid Sequence Chromatography, High Pressure Liquid Cosyntropin - analysis Cosyntropin - chemical synthesis
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Summary:	The applicability of reversed-phase ion-pair HPLC for separating the ACTH-(1-24) fragments obtained at the final stage of the synthesis has been examined. Optimization of chromatographic conditions (selection of proper packing material, eluent and concentration of ion-pairing reagent) for separation of protected peptides has been performed. A mixture of the ACTH fragments 1-4, 5-12, 13-24, 5-24, and 1-24 has been resolved on a Zorbax C8 column using methanol - 0,01 M tetrabutylammonium bromide (94:6) as mobile phase.
ISSN:	0132-3423