Regulation of cholesterol absorption in human and rat small intestine epithelial cells

Cholesterol absorption in human small intestine organ culture and rat small intestine epithelial cell culture IRD-98 has been studied using [14C] cholesterol, [3H] cholesterol and [14C] sitosterol. It has been found that cholesterol absorption is a dose- and time-dependent process, while sitosterol...

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Bibliographic Details
Published in:	Biokhimiia (Moscow, Russia) Vol. 58; no. 2; p. 274
Main Authors:	Safonova, I G, Sviridov, D D, Rampal', P, Nano, Zh L, Pavlov, M Iu, Repin, V S
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-02-1993
Subjects:	Adolescent Animals Cells, Cultured Child Child, Preschool Cholesterol - metabolism Enzyme Inhibitors - pharmacology Epithelial Cells Epithelium - metabolism Epithelium - physiology Humans Infant Infant, Newborn Intestinal Absorption - drug effects Intestine, Small - cytology Intestine, Small - metabolism Intestine, Small - physiology Organ Culture Techniques Rats Sitosterols - metabolism
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Summary:	Cholesterol absorption in human small intestine organ culture and rat small intestine epithelial cell culture IRD-98 has been studied using [14C] cholesterol, [3H] cholesterol and [14C] sitosterol. It has been found that cholesterol absorption is a dose- and time-dependent process, while sitosterol absorption is not and makes up to about 25% of the total cholesterol absorption. Cholesterol absorption appeared to be a specific process. The endocytosis inhibitor monensin decreased specific cholesterol absorption by 37%. Cholesterol absorption was examined under different conditions influencing cholesterol metabolism in the cell. Loading of IRD-98 cells with non-lipoprotein cholesterol caused a dose-dependent decrease of cholesterol absorption. The inhibitor of acyl coenzyme A:cholesterol acyl transferase (ACAT), compound Sandoz 58-035, had a similar effect on cholesterol absorption. Lovastatin, an inhibitor of 3-hydroxymethyl-3-glutaryl coenzyme A (HMG-CoA) reductase, stimulated cholesterol absorption in a dose-dependent manner. Loading of cells with cholesterol, lovastatin and Sandoz 58-035 had no effect on sitosterol absorption. The possibility has been demonstrated of using human small intestine organ culture and rat small intestine epithelial cell culture IRD-98 as models for studying cholesterol absorption.
ISSN:	0320-9725