Simple chromatographic determination of the neopterin marker in immunopathies

Estimation of neopterin in urine has become part of examination in phenylketonuria, malignities, immunodeficiency incl. AIDS and HIV infection. The authors describe a simple chromatographic method for estimation of neopterin which does not call for highly efficient liquid chromatography nor imported...

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Bibliographic Details
Published in:	Časopis lékařů českých Vol. 128; no. 2; p. 59
Main Authors:	Tomsová, Z, Jůzová, Z
Format:	Journal Article
Language:	Czech
Published:	Czech Republic 06-01-1989
Subjects:	Acquired Immunodeficiency Syndrome - diagnosis Biomarkers - urine Biopterins - analogs & derivatives Biopterins - urine Chromatography, Thin Layer - methods Humans Immunologic Deficiency Syndromes - diagnosis Neoplasms - diagnosis Neopterin
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Summary:	Estimation of neopterin in urine has become part of examination in phenylketonuria, malignities, immunodeficiency incl. AIDS and HIV infection. The authors describe a simple chromatographic method for estimation of neopterin which does not call for highly efficient liquid chromatography nor imported kits. The di- and tetrahydro- forms of neopterin are oxidized with MnO2 and stable neopterin is obtained. The specimen is purified on a Dowex 50WX4 (Fluka) column in a H+ cycle and on PRESEPC18 (TESSEK) columns. Then thin-layer chromatography on Merck Kieselgel 60F254 plates is used in a system of ethyl acetate--isopropanol--1 N NH4OH (35:45:25). Fluorescence is assessed on a densitometer (Shimadzu CS-920 at 367.5 nm). The RF of neopterin is 0.214. The fluorescence is linear within the range of 5-200 ng. The method is sufficiently sensitive also for the estimation of normal neopterin excretion. The authors submit the results of estimations in controls, immunopathies and AIDS.
ISSN:	0008-7335