The isolation and study of the characteristics of a cytopathic strain of the hepatitis A virus

A fast-growing cytopathic isolate of human hepatitis A virus (strain MB-7) was derived from fecal samples of infected patients and adapted to growth in FRhK-4 cell culture. A positive serum standard against HAV and electron microscopy were used to demonstrate that MB-7 belonged to human hepatitis A...

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Bibliographic Details
Published in:	Voprosy virusologiĭ Vol. 38; no. 3; p. 101
Main Authors:	Maĭdaniuk, A G, Tiunnikov, G I, Konakova, V E, Bondarenko, E P, Nemtsov, Iu V, Karpovich, L G, Kalashnikova, T V, Mamaeva, N V, Sosnovtsev, S V, Chizhikov, V E
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-05-1993
Subjects:	Antigens, Viral - analysis Base Sequence Cloning, Molecular Cytopathogenic Effect, Viral DNA, Complementary - genetics DNA, Viral - genetics Hepatitis A - microbiology Hepatovirus - genetics Hepatovirus - growth & development Hepatovirus - immunology Hepatovirus - isolation & purification Hepatovirus - pathogenicity Humans Microscopy, Electron Molecular Sequence Data Neutralization Tests Siberia Virus Cultivation Siberia
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Summary:	A fast-growing cytopathic isolate of human hepatitis A virus (strain MB-7) was derived from fecal samples of infected patients and adapted to growth in FRhK-4 cell culture. A positive serum standard against HAV and electron microscopy were used to demonstrate that MB-7 belonged to human hepatitis A virus. The strain MB-7 induced plaque formation in FRhK-4 under agar overlay after 10-12 days of incubation. The PCR products of gene VP1 were cloned in E. coli and its primary structure was determined. MB-7 was shown to have more homology with HAV strains isolated in the USA and China.
ISSN:	0507-4088