Criteria of the sensitivity of Zajdela and Morris hepatoma cells to glucocorticoids

Glucocorticoids induce tyrosine aminotransferase synthesis in 7777 Morris hepatoma but fail to do so in Zajdela hepatoma. This internal property indicates the resistance to the hormone. However, both hepatoma cell lines do respond to the triamcinolone acetonide in a similar way, as judged by some ot...

Full description

Saved in:

Bibliographic Details
Published in:	Biulleten' eksperimental'noi biologii i meditsiny Vol. 104; no. 11; p. 615
Main Authors:	Dmitrieva, L V, Neustroeva, N P, Polotskaia, A V, Sturchak, S V, Shapot, V S
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-11-1987
Subjects:	Animals Cell Line Enzyme Induction - drug effects Glucocorticoids - pharmacology In Vitro Techniques Liver Neoplasms, Experimental - enzymology Liver Neoplasms, Experimental - pathology Neoplasms, Hormone-Dependent Tyrosine Transaminase - metabolism
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Glucocorticoids induce tyrosine aminotransferase synthesis in 7777 Morris hepatoma but fail to do so in Zajdela hepatoma. This internal property indicates the resistance to the hormone. However, both hepatoma cell lines do respond to the triamcinolone acetonide in a similar way, as judged by some other criteria, e. g. interaction with the immobilized hormone on the inert carrier, adhesion to glass and kinetic parameters of alkaline phosphodiesterase I activity. Moreover, both cell types respond to glucocorticoids by modification of synthesis of some proteins, as revealed previously by two-dimensional electrophoresis. The results show that in case of tumour cells which retain their specific receptor apparatus but do not respond to glucocorticoids by usual criteria, the conclusion whether tumour cells are hormone-sensitive or not has to be drawn from the analysis of their multiple response judging by several assays.
ISSN:	0365-9615