Pyruvate kinase isoenzymes in the kidneys of rabbits with insular insufficiency

Two isoenzymes of pyruvate kinase--PK-1 and PK-2 were obtained from the cortical layer of rabbit kidney by the method of chromatography on DEAE-cellulose. Starvation of rabbits for 10--16 days and alloxan diabetes produced no significant changes in the specific activity of PK in the soluble fraction...

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Bibliographic Details
Published in:	Problemy ėndokrinologii Vol. 21; no. 2; p. 78
Main Authors:	Dozhevnikova, K A, Il'in, V S
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-03-1975
Subjects:	Animals Chromatography, DEAE-Cellulose Colorimetry Diabetes Mellitus, Experimental - metabolism Isoenzymes - metabolism Kidney Cortex - enzymology Male Pyruvate Kinase - metabolism Rabbits Starvation - metabolism Time Factors
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Summary:	Two isoenzymes of pyruvate kinase--PK-1 and PK-2 were obtained from the cortical layer of rabbit kidney by the method of chromatography on DEAE-cellulose. Starvation of rabbits for 10--16 days and alloxan diabetes produced no significant changes in the specific activity of PK in the soluble fraction obtained from the cortical layer of rabbit kidney. However, there were significant shifts in the isoenzymatic spectrum of the PK of the kidneys in rabbits with alloxan diabetes: the activity of the PK-1 increased considerably and significantly, and the isoenzyme PK-2 disappeared almost completely.
ISSN:	0375-9660