Expression in Escherichia coli cells of mutant human interferon alpha2

cDNA library was obtained from mRNA isolated from human leukocytes induced by Newcastle disease virus. Clones containing cDNA for alpha 2-interferons were identified by colony hybridization with two synthetic hexadecanucleotides. One of the positive clones contained a NH2-terminal part of cDNA of hu...

Full description

Saved in:

Bibliographic Details
Published in:	Molekuliarnaia biologiia Vol. 18; no. 1; p. 48
Main Authors:	Ovchinnikov, Iu A, Sverdlov, E D, Monastyrskaia, G S, Tsarev, S A, Zaĭtseva, E M
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-01-1984
Subjects:	Animals Autoradiography Base Sequence Cells, Cultured Chromosome Mapping Cloning, Molecular DNA - analysis DNA - genetics DNA, Bacterial - analysis DNA, Bacterial - genetics Escherichia coli - genetics Gene Expression Regulation Genes Humans Interferon Type I - genetics Mice Mutation Operon Plasmids Radioimmunoassay RNA, Messenger - analysis RNA, Messenger - genetics
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	cDNA library was obtained from mRNA isolated from human leukocytes induced by Newcastle disease virus. Clones containing cDNA for alpha 2-interferons were identified by colony hybridization with two synthetic hexadecanucleotides. One of the positive clones contained a NH2-terminal part of cDNA of human interferon identical to cDNA for IFN-alpha 2. The only difference between these two clones was the Ser-8 leads to Asn-8 substitution in deduced sequenced of mature interferons. This mutant interferon, named alpha 2, was expressed in E. coli and its properties were compared with those of interferon alpha 2.
ISSN:	0026-8984