Isolation and study of some properties of new phosphopeptides from nerve tissue

The phosphopeptides were extracted by organic solvents from the lipid-deleted homogenate of rat brain tissue. The latter was obtained by means of trichloroacetic acid from rats injected with 32P-NaH2PO4 into brain ventricles. Chromatography of the brain extract on DEAE-Sephadex A-25 and gel filtrati...

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Bibliographic Details
Published in:	Biokhimiia (Moscow, Russia) Vol. 47; no. 7; p. 1125
Main Authors:	Mikeladze, D G, Kacharava, D G
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-07-1982
Subjects:	Amino Acids - analysis Animals Brain Chemistry Chromatography, Gel Chromatography, Ion Exchange Molecular Weight Phosphopeptides - isolation & purification Rats
Online Access:	Get more information
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Summary:	The phosphopeptides were extracted by organic solvents from the lipid-deleted homogenate of rat brain tissue. The latter was obtained by means of trichloroacetic acid from rats injected with 32P-NaH2PO4 into brain ventricles. Chromatography of the brain extract on DEAE-Sephadex A-25 and gel filtration through Sephadex G-25 and G-15 resulted in 3 homogenous fractions of the phosphopeptides characterized by the maximal rate of the label incorporation. These fractions appeared to be homogenous during polyacrylamide gel electrophoresis and thin-layer chromatography. The molecular weights of the fractions are equal to 1200, 1100 and 900, respectively; the isoelectric points lie at 3.2, 3.0 and 3.1, respectively. All the phosphopeptides tested contain large amounts of glycine, serine and glutamic acid and have alanine as N-terminal amino acid.
ISSN:	0320-9725