Detection and identification of new cardioactive proteins in cattle heart

Seven cardioactive polypeptides were identified in precardiac and auricular regions of bovine heart. Procedures used for purification of the polypeptides involved extraction of water-soluble proteins, fractionation by ammonium sulfate, gel filtration on Sephadex G-100, chromatography and rechromatog...

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Bibliographic Details
Published in:	Voprosy meditsinskoi khimii Vol. 37; no. 2; p. 56
Main Authors:	Popova, T V, Srapionian, R M, Galoian, A A
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-03-1991
Subjects:	Amino Acids - analysis Animals Cattle Chromatography, Liquid Coronary Vessels - drug effects Molecular Weight Muscle Proteins - isolation & purification Muscle Proteins - pharmacology Muscle Proteins - physiology Myocardium - chemistry
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Summary:	Seven cardioactive polypeptides were identified in precardiac and auricular regions of bovine heart. Procedures used for purification of the polypeptides involved extraction of water-soluble proteins, fractionation by ammonium sulfate, gel filtration on Sephadex G-100, chromatography and rechromatography on DEAE cellulose. Some physico-chemical and biological properties were studied; amino acid composition, N-terminal amino acids, molecular mass and the effects on coronary blood vessels. The data obtained suggest that multiple forms of these proteins may exist either as a result of partial hydrolysis of large molecules or due to processing of precursors.
ISSN:	0042-8809