New findings concerning cardiac proteins binding the coronary dilating substances of the hypothalamus

We had previously shown that the protein component obtained from heart muscle by 0.1--0.2 saturation with ammonium sulfate exhibits a coronarodilatatory effect. Later on we succeeded in isolating from this protein complex, through dialysis, two coronaoractive low molecular compounds, one of which is...

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Bibliographic Details
Published in:	Voprosy biokhimii mozga Vol. 10; p. 122
Main Authors:	Srapionian, R M, Misirian, S S, Galoian, A A
Format:	Journal Article
Language:	Russian
Published:	Armenia (Republic) 1975
Subjects:	Animals Carrier Proteins - isolation & purification Cattle Chemical Phenomena Chemistry Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Hypothalamus - analysis Myocardium - analysis Spectrum Analysis Swine Vasodilator Agents - metabolism
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Summary:	We had previously shown that the protein component obtained from heart muscle by 0.1--0.2 saturation with ammonium sulfate exhibits a coronarodilatatory effect. Later on we succeeded in isolating from this protein complex, through dialysis, two coronaoractive low molecular compounds, one of which is apparently a neurohormone. In the present study we isolated, purified and studied the features of this protein complex. The results of gel filtration on Sephadex G-100 confirmed the presence of two different coronaroactive neurohormone binding proteins in heart muscle. The active fractions have been also purified through ion exchange chromatography on diethylaminoethyl cellulose. The purity of these fractions was tested through electrophoresis on 7.5% polyacrylamide gel. In the last stage of purification the active proteins moved towards the anode by one zone.
ISSN:	0507-2972