Comparison of protein concentrations in saliva and serum

• Published in: Annales de biologie clinique (Paris) Vol. 46; no. 6; p. 361
• Main Authors: Grimoud, A M, Pontet, F, Rousselet, F, Lodter, J P, Duffaut, D, Seguela, J P
• Format: Journal Article
• Language: French
• Published: France 1988
• Subjects: Adult; Age Factors; Albumins - analysis; alpha 1-Antitrypsin; alpha-Macroglobulins - analysis; Analysis of Variance; Blood Proteins - analysis; C-Reactive Protein - analysis; Complement C3 - analysis; Complement C3c; Complement C4 - analysis; Female; Haptoglobins - analysis; Humans; Immunoglobulin A - analysis; Immunoglobulin G - analysis; Immunoglobulin M - analysis; Male; Middle Aged; Nephelometry and Turbidimetry; Proteins - analysis; Saliva - analysis; Saliva - immunology; Serum Albumin - analysis; Sex Factors; Transferrin - analysis
• ISSN: 0003-3898

Saliva and serum protein profiles composed of 12 proteins: IgA, IgG, IgM, albumin, transferrin, alpha 1-glycoprotein acid, alpha 2-macroglobulin, alpha 1-proteinase inhibitor, haptoglobine, C3C, C4 and CRP were obtained by laser nephelometry of aliquots of samples of non-stimulated whole saliva and blood taken at the same time. The population of 138 controls of both sexes was divided into 4 age groups: 1 (20 to 29 years), 2 (30 to 39 years), 3 (40 to 49 years), 4 (50 to 60 years). Using the techniques developed for saliva assay, 10 proteins were measurable: IgA, IgG, IgM, albumin, transferrin, alpha 1-glycoprotein acid, alpha 2-macroglobulin, alpha 1-proteinase inhibitor, haptoglobulin and C3C. The C4 fraction and CRP were present at concentrations lower than the detection limit i.e. at 1.8 and 2.05 mg/l. In the serum 11 proteins were analysed, CRP occurring at less than 0.01 g/l. The statistical two-factor variance analysis test (sex and age) demonstrated the statistically significant influence of age in the saliva for alpha 1-PI and alpha 2-M in the serum for Tf, alpha 2-M and alpha 1-PI, and sex in the serum for IgG, IgM, alpha 1-PI, alpha 2-M and also the interaction of both factors in the serum for alpha 2-M.