Isozymes of NADP-dependent isocitrate dehydrogenase in normal and tumor tissues of various mouse strains and their hybrids

• Published in: T͡S︡itologii͡a Vol. 27; no. 7; p. 837
• Main Authors: Fedorova, E V, Vakhtin, Iu B
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-07-1985
• Subjects: Alleles; Animals; Crosses, Genetic; Electrophoresis, Polyacrylamide Gel; Genes; Genetic Variation; Isocitrate Dehydrogenase - analysis; Isocitrate Dehydrogenase - genetics; Isoenzymes - analysis; Isoenzymes - genetics; Methylcholanthrene; Mice; Mice, Inbred Strains - metabolism; Neoplasm Transplantation; Neoplasms, Experimental - chemically induced; Neoplasms, Experimental - enzymology; Rhabdomyosarcoma - enzymology
• ISSN: 0041-3771

Normal tissues of DBA, CBA, CC57W, C3H, Balb/c, SHR mice and F1 hybrids CC57W/DBA appeared to differ in the ratios of mitochondrial and supernatant NADP-dependent isocitrate dehydrogenase (IDH). Tested inbred mice strains CC57W, C3H, SHR, Balb/c contain allelic form Idh-1a of supernatant IDH gene Idh-1, whereas allelic form Idh-1b is characteristic of mice strains DBA and CBA. In tumors IDH isozymes have the same mobility as do isozymes of homologous normal tissues; but their activity is lower. A high variability of each isozyme activity in the isozyme spectrum is revealed in various tissues of F1 hybrids CC57W/DBA. Allelic forms of gene Idh-1 were used as markers of normal and tumor cells for the experimental model: transplantation of sarcoma 37 (Idh-1a/Idh-1a) to subcutaneous tissue of the mouse strain DBA (Idh-1b/Idh-1b). It enables us to reveal isozymes of stromal cell in tumor IDH isozyme spectrum. The results indicate that the relation of normal and tumor isozymes vary in different tumors.