N- and C-terminal amino acids of proteolipids from the rat brain and various other organs

• Published in: Ukrainskij biohimičeskij žurnal Vol. 60; no. 3; p. 71
• Main Authors: Kazarian, T I, Manukian, K G
• Format: Journal Article
• Language: Russian
• Published: Ukraine 01-05-1988
• Subjects: Amino Acids - analysis; Animals; Brain Chemistry; Chromatography, Thin Layer; Kidney - analysis; Liver - analysis; Muscles - analysis; Myocardium - analysis; Organ Specificity; Proteolipids - analysis; Rats
• ISSN: 0201-8470

N- and C-terminal amino acids of proteolipid proteins from the whole brain and some other organs were investigated. N-terminal amino acids were identified by the dansylation procedure. C-terminal amino acids were determined after the enzymatic hydrolysis with carboxy peptidases A and B with the following dansylation. Phenyl alanine and lysine were identified as C-terminal amino acids of the proteolipids from the whole brain and only lysine--as the C-terminal amino acid of proteolipids from the heart, liver, kidney (cortical and medullary parts) and skeletal muscle. The corresponding N-terminal amino acids of the proteolipids from the whole brain were aspartic acid and glycine and of proteolipids from the heart, liver, kidney (cortical and medullary parts) and skeletal muscle--only aspartic acid. A comparison of the data obtained with the previous ones has shown that in the brain there exist only two types of proteolipids--one characteristic of myelin, another-- of mitochondria, and in other organs--only one characteristic of mitochondria.