The oxygen system of an isolated heart during its perfusion with a suspension of human erythrocytes after different periods of their preservation in a solution containing adenine

• Published in: Gematologiia i transfuziologiia Vol. 36; no. 8; p. 18
• Main Authors: Iarochkin, V S, Vinogradov, S M, Agranenko, V A, Suvorova, I A, Tibilova, N N
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-08-1991
• Subjects: Adenine - administration & dosage; Adenine - pharmacology; Animals; Anticoagulants - administration & dosage; Blood Preservation - methods; Citrates - administration & dosage; Citrates - pharmacology; Cryopreservation - methods; Cryoprotective Agents; Erythrocytes - drug effects; Erythrocytes - physiology; Glucose - administration & dosage; Glucose - pharmacology; Heart Arrest, Induced; Humans; Hypoxia - prevention & control; Male; Models, Cardiovascular; Myocardium - metabolism; Oxygen - blood; Oxygen Consumption - drug effects; Oxygen Consumption - physiology; Perfusion - methods; Rats; Rats, Inbred Strains; Solutions; Time Factors
• ISSN: 0234-5730

Seven series of experiments (68 tests) were conducted on an isolated rat heart with the functioning left ventricle during its coronary vessels perfusion with suspension of human erythrocytes of different storage terms preserved in the solution containing citroglucophosphate with adenine. The main parameters of hemodynamics and oxygen regimen were evaluated. The results of the study have shown that washed human donor erythrocytes preserved in the above solution after 1, 7, 14, 21 and 28 days of storage present a highly efficient gas-carrying medium under conditions of acute anemic hypoxia.