Protein substrates of proteinases with fluorescent labels

Protein substrates for proteinases with double fluorescent fluorophors are synthesized. Pyridoxal-5'-phosphate, dansyl chloride and fluorescein isothiocyanate were used as fluorescent sounds for modification of globin. Phosphoyridoxyl fluorophor was present in the both substrates. The second la...

Full description

Saved in:

Bibliographic Details
Published in:	Ukrainskij biohimičeskij žurnal Vol. 60; no. 2; p. 11
Main Authors:	Kalachian, A S, Mnatsakanian, G A, Akopian, T N, Arutiunian, A A
Format:	Journal Article
Language:	Russian
Published:	Ukraine 01-03-1988
Subjects:	Affinity Labels Animals Cattle Endopeptidases - isolation & purification Fluorescent Dyes - chemical synthesis Hydrolysis Rats Spectrometry, Fluorescence Substrate Specificity
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Protein substrates for proteinases with double fluorescent fluorophors are synthesized. Pyridoxal-5'-phosphate, dansyl chloride and fluorescein isothiocyanate were used as fluorescent sounds for modification of globin. Phosphoyridoxyl fluorophor was present in the both substrates. The second label was either fluoresceinthiocarbamyl or dansyl fluorophor. Spectral characteristics and ability to hydrolysis of obtained substrates have been studied. The influence of some salts on fluorescent characteristics of those substrates have been analyzed. Differentiation of the hydrolyzed substrate from the initial one by ammonium sulphate is shown to be possible.
ISSN:	0201-8470