Study of the structural lability of biomembranes and their components by the fluorescent analysis method. IV. Kinetic characteristics of the binding of unsaturated fatty acids with bovine serum albumin

By studying fluorescent parameters of ionic and neutral probes the kinetics of complex formation between the molecules of bovine serum albumin (BSA) and unsaturated fatty acids was investigated. The following regularities were observed: 1. Unlike the saturated fatty acids the unsaturated ones change...

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Bibliographic Details
Published in:	Biofizika Vol. 21; no. 6; p. 1013
Main Authors:	Danilov, V S, Orlov, S N, Rebrov, V G, Fedin, V A, Shevchenko, A S
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-11-1976
Subjects:	Anilino Naphthalenesulfonates Arachidonic Acids Fatty Acids, Unsaturated Fluorescence Linoleic Acids Naphthalenes Oleic Acids Protein Binding Serum Albumin, Bovine Temperature
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Summary:	By studying fluorescent parameters of ionic and neutral probes the kinetics of complex formation between the molecules of bovine serum albumin (BSA) and unsaturated fatty acids was investigated. The following regularities were observed: 1. Unlike the saturated fatty acids the unsaturated ones changed fluorescent parameters of the probes according to the cooperative mechanism, significantly decreasing the quantum yield values; 2. Temperature dependence of the fluorescence of both probe types in the complexes BSA -- oleic acid and BSA -- linole acid shows some bends at t=30--39 degrees C for which the lipid components is responsible.
ISSN:	0006-3029