Radioimmunological characteristics of the distribution of hypothalamic glycoprotein--a precursor of cardiotropic neurohormone C--in the brain and viscera

• Published in: Voprosy meditsinskoi khimii Vol. 31; no. 2; p. 20
• Main Authors: Srapionian, R M, Abelian, Zh G, Galoian, A A
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-03-1985
• Subjects: Animals; Brain - metabolism; Brain Chemistry; Carrier Proteins - analysis; Cats; Cattle; Glycoproteins - analysis; Hypothalamic Hormones - metabolism; Hypothalamus - analysis; Hypothalamus - metabolism; Immunization; Protein Precursors - analysis; Rabbits; Radioimmunoassay - methods; Solubility; Tissue Distribution
• ISSN: 0042-8809

A radioimmunological procedure is described for estimation of cardiotropic glycoprotein (BNS), isolated from bovine hypothalamus, in various brain structures and some visceral tissues. The rabbit antiserum towards bovine BNS was used at a dilution 1:10,000. Concentration of BNS measured by the procedure in cat hypothalamus and cerebellum was equal to 1.5 ng and 0.84 ng/g of wet tissue, respectively. The immunoreactive activity of BNS was also found in adrenal glands, heart and skeletal muscles; it was not observed in kidney, lung and pancreas. Content of BNS in cat blood was 10 ng/ml. BNS appears to penetrate into circulating blood.