The effect of hypoxia and of subsequent baro-oxygenation on the function of the microsomal oxidation system in the rat liver

• Published in: Nauchnye doklady vysshei shkoly. Biologicheskie nauki no. 7; p. 86
• Main Authors: Shchugaleĭ, V S, Ananian, A A, Miliutina, N P, Sadekova, S I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 1992
• Subjects: Altitude; Animals; Antioxidants; Atmosphere Exposure Chambers; Cell Membrane - metabolism; Cytochrome P-450 Enzyme System - metabolism; Hyperbaric Oxygenation; Hypoxia - metabolism; Lipid Bilayers - metabolism; Lipid Peroxidation; Male; Microsomes, Liver - metabolism; Oxidation-Reduction; Rats; Viscosity
• ISSN: 0470-4606

The increase of cytochrome P-450 by 34% and its catalytic activity with substrate amidopyrine by 57% as compared with control has been shown under hypoxia (0.029 MPa, 1 h). Hyperoxia (0.2 MPa, 1 h) increases the metabolism of amidopyrine by 148%, benzo[a]pyrene by 158% and aniline by 114% and consecutive affection of hypoxia and hyperoxia--by 247, 45 and 138% correspondingly at fixed cytochrome P-450 amount in both series. The amount of diene conjugates and Schiff's bases under hypoxia increases by 40 and 69% correspondingly, the activity of SOD and catalase decreases by 25 and 23%. The activity of hyperoxia raises the diene conjugate content by 19% at all this SOD activity increases by 95%. Consecutive affection of hypoxia and hyperoxia increases the level of diene conjugates and Schiff's bases by 26 and 23% correspondingly, without changing SOD and catalase activity. The relative microsomal viscosity of lipid layer and zones of enzyme-lipid contacts decreased by 20 and 24% under hypoxia, but under hyperoxia and consecutive affection and hypoxia and hyperoxia it increases by 29-28% and 56-40% correspondingly.