Quantitative determination of the infective activity of the human immunodeficiency virus by plaque formation using monolayer cultures

Quantitative determination of the infective activity of the human immunodeficiency virus by plaque formation using monolayer cultures

A method for assessment of the infective activity of HIV using plaque formation method with human diploid cells (strains L-65, L-68, L-72) has been developed. This method is not inferior to plaque formation with poly-L-lysine in sensitivity, but superior to it in reproducibility of results and stand...

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Bibliographic Details
Published in:Voprosy virusologiĭ Vol. 40; no. 5; p. 202
Main Authors: Nosik, M N, Stepanova, L G, Matsevich, G R
Format: Journal Article
Language:Russian
Published: Russia (Federation) 01-09-1995
Subjects:
Antibodies, Viral - immunology
Cell Line
HIV - immunology
HIV - pathogenicity
HIV - physiology
Humans
Neutralization Tests
Reproducibility of Results
Viral Plaque Assay
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Summary:A method for assessment of the infective activity of HIV using plaque formation method with human diploid cells (strains L-65, L-68, L-72) has been developed. This method is not inferior to plaque formation with poly-L-lysine in sensitivity, but superior to it in reproducibility of results and standardization of the test conditions. The method is fit for titration of HIV and virus-neutralizing antibodies, as well as for assessment of the activity of anti-HIV preparations.
ISSN:0507-4088