Glutathione disulfide inhibits degradation of substance P induced by synaptosomal plasma membranes

The effects of phosphorylation, ribosylation of proteins and formation of protein-mixed disulfides on substance P degradation under the action of synaptosomal plasma membranes were studied. It was found that only the formation of mixed disulfides between membrane proteins and oxidized glutathione af...

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Bibliographic Details
Published in:	Biokhimiia (Moscow, Russia) Vol. 54; no. 12; p. 2067
Main Authors:	Akopian, T N, Arzumanian, A M, Agadzhanian, A G, Arutiunian, A A
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-12-1989
Subjects:	Animals Brain - metabolism Cell Fractionation Cell Membrane - metabolism Glutathione - analogs & derivatives Glutathione - pharmacology Glutathione Disulfide Membrane Proteins - metabolism Rats Substance P - antagonists & inhibitors Substance P - metabolism Synaptic Membranes - metabolism
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Summary:	The effects of phosphorylation, ribosylation of proteins and formation of protein-mixed disulfides on substance P degradation under the action of synaptosomal plasma membranes were studied. It was found that only the formation of mixed disulfides between membrane proteins and oxidized glutathione affected (inhibited) the peptide degradation process. Using an oxidized glutathione fluorescent derivative, it was shown that a 50% inhibition occurs as a result of binding of 2 nmol of the glutathione residue to 1 mg of the membrane protein.
ISSN:	0320-9725