Influenza viruses studied by fluorescent immunoassay with temporary resolution

Monoclonal antibodies, epidemic strains of influenza A and B viruses, conjugates were studied by fluorescence immunoassay with temporary resolution using equipment of different companies. Differences and specificity of monoclonal antibodies to influenza A and B viruses were demonstrated. The highest...

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Bibliographic Details
Published in:	Voprosy virusologiĭ Vol. 35; no. 2; p. 115
Main Authors:	Ivanova, V T, Ponomareva, I V, Shakhanina, K L, Iakhno, M A, Slepushkin, A N, Savitskiĭ, A P
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-03-1990
Subjects:	Antibodies, Monoclonal Antibodies, Viral - analysis Antibody Specificity - immunology Evaluation Studies as Topic Fluorescent Antibody Technique - instrumentation Influenza A virus - immunology Influenza B virus - immunology Viral Core Proteins - immunology
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Summary:	Monoclonal antibodies, epidemic strains of influenza A and B viruses, conjugates were studied by fluorescence immunoassay with temporary resolution using equipment of different companies. Differences and specificity of monoclonal antibodies to influenza A and B viruses were demonstrated. The highest sensitivity of the method with the test system used was determined, being 20 ng/ml of viral protein. The method was shown to be useful for influenza virus detection both in solutions containing purified virions and in virus-containing allantoic preparations. It was established that virological studies could be carried out using conjugates and knock-down microplates of the national make alongside with foreign ones.
ISSN:	0507-4088