Interrelationship between the chain length of poly-L-lysine and the degree of protection of polyribonucleotide interferon inducers from human blood nucleases

• Published in: Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic] Vol. 38; no. 7; p. 21
• Main Authors: Surzhik, M A, Duks, A E, Diatlova, N G, Glazunov, E A, Feldmane, G Ia, Timkovskiĭ, A L
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-07-1993
• Subjects: Humans; Interferon Inducers - chemistry; Interferon Inducers - pharmacology; Kinetics; Molecular Weight; Organic Chemicals; Polylysine - chemistry; Ribonucleases - blood; RNA, Double-Stranded - chemistry; RNA, Double-Stranded - pharmacology; RNA, Fungal - chemistry; RNA, Fungal - pharmacology
• ISSN: 0235-2990

The action of the human total blood serum on polynucleotide interferon inducers, larifan and ridostin (natural double-stranded RNAs) and poly(I).poly(C) (a double-stranded complex of synthetic polyribonucleotides) used both in the free state and in the state shielded with poly-L-lysine was studied. The rate of the accumulation of the acid soluble products was compared with the residual interferon-inducing activity in mice. All the unshielded inducers were shown to completely loose their activity after a 4-hour contact with the serum. The protective activity of poly-L-lysine increased in parallel with the increase of its molecular weight and was maximal for the preparation with the molecular weight of 12300 +/- 1000 Da. Differences in the structure of the inducers and the mechanism of their biosynthesis and degradation must be taken into account.