Expression of hybrid genes containing sequences coding for human adrenocorticotropic hormone in Escherichia coli strains

E. coli strains producing a hybrid protein containing human adrenocorticotropic hormone (ACTH) and protein A of S. aureus were obtained. The sequence coding for ACTG was obtained from the bovine one using oligonucleotide-directed mutagenesis. The ACTG gene was linked with the protein A gene and its...

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Bibliographic Details
Published in:	Molekuliarnaia biologiia Vol. 28; no. 3; p. 595
Main Authors:	Karapetian, V E, Parsadanian, A Sh, Galoian, A A
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-05-1994
Subjects:	Adrenocorticotropic Hormone - genetics Animals Cattle Chromatography, Affinity Chromatography, High Pressure Liquid Cloning, Molecular DNA, Recombinant Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Humans Plasmids Recombinant Fusion Proteins - genetics
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Summary:	E. coli strains producing a hybrid protein containing human adrenocorticotropic hormone (ACTH) and protein A of S. aureus were obtained. The sequence coding for ACTG was obtained from the bovine one using oligonucleotide-directed mutagenesis. The ACTG gene was linked with the protein A gene and its derivatives by synthetic adaptors. It was shown that each of the constructed plasmids directed the synthesis of hybrid protein in E. coli. This protein was purified on IgG-Sepharose. Then ACTH was obtained by HPLC after specific hydrolysis. The amino acid composition of purified sample was determined.
ISSN:	0026-8984