Mutagenesis, induced by phosphotriester analogs of oligonucleotides and directed to the cleavage site of double-spiral DNA

The mutagenic properties of phosphotriester analogues revealed in course of interaction with linearized plasmid DNA were studied. The plasmid-based model system permitting one to test reliably the induced mutations is proposed. The efficiency of mutagenesis was shown to depend on the length of the o...

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Bibliographic Details
Published in:	Molekulârnaâ genetika, mikrobiologiâ i virusologiâ no. 10; p. 19
Main Authors:	Petrenko, V A, Karpenko, L I, Tat'kov, S I, Smirnova, O Iu, Kuz'micheva, G A, Il'ichev, A A
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-10-1991
Subjects:	Bacteriophages - genetics Base Sequence DNA, Bacterial - genetics DNA, Viral - genetics Escherichia coli - genetics Esters - chemistry Genes, Bacterial Genes, Viral Molecular Sequence Data Mutagenesis, Site-Directed Oligonucleotides - chemistry Organophosphorus Compounds - chemistry
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Summary:	The mutagenic properties of phosphotriester analogues revealed in course of interaction with linearized plasmid DNA were studied. The plasmid-based model system permitting one to test reliably the induced mutations is proposed. The efficiency of mutagenesis was shown to depend on the length of the oligonucleotide-mutagen and the genotype of the transformed Escherichia coli strain. The possible mechanisms involved in mutagenesis are discussed.
ISSN:	0208-0613