Comparative analysis of the structure of SUP2 genes in Pichia pinus and Saccharomyces cerevisiae

SUP2(SUP35) is an omnipotent suppressor gene, coding for an EF-1 alpha-like protein factor, involved in the control of translational accuracy in yeast Saccharomyces cerevisiae. A SUP2 gene analogue from yeast Pichia pinus was isolated by complementation of temperature-sensitive sup2 mutation of S. c...

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Bibliographic Details
Published in:	Molekuliarnaia biologiia Vol. 24; no. 4; p. 1024
Main Authors:	Kushnirov, V V, Ter-Avanesian, M D, Smirnov, V N, Chernov, Iu O, Derkach, I L, Novikova, O N, Inge-Vechtomov, S G, Neĭstat, M A, Tolstorukov, I I
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-07-1990
Subjects:	Amino Acid Sequence Base Sequence Codon Fungal Proteins - genetics Genes, Fungal Molecular Sequence Data Pichia - genetics Restriction Mapping Saccharomyces cerevisiae - genetics Sequence Homology, Nucleic Acid Species Specificity Suppression, Genetic
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Summary:	SUP2(SUP35) is an omnipotent suppressor gene, coding for an EF-1 alpha-like protein factor, involved in the control of translational accuracy in yeast Saccharomyces cerevisiae. A SUP2 gene analogue from yeast Pichia pinus was isolated by complementation of temperature-sensitive sup2 mutation of S. cerevisiae. Nucleotide sequence of the SUP2 gene of P. pinus codes for a protein of 82.4 kDa exceeding the SUP2 protein of S. cerevisiae for 6 kDa. The SUP2 gene product of P. pinus is similar to the Sup2 protein of S. cerevisiae by its structure and includes a highly conservative (76%) C-terminal region homologus to EF-1 alpha and a lowly conservative N-terminal region. The relation between the evolutionary conservativity of different regions of the Sup2 protein and their functional significance is discussed.
ISSN:	0026-8984