Soluble cerebral metalloproteins. 1. Purification and properties of cerebral cortex cytochrome c

Cytochrome c from grey matter of brain has been obtained as a homogeneous preparation by electrophoresis on polyacrylamide gel and following electrofocusing in ampholine solutions. Its molecular weight, content of iron, redox potential and isoelectric point have been established. The absolute spectr...

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Bibliographic Details
Published in:	Voprosy biokhimii mozga Vol. 10; p. 66
Main Authors:	Sharoian, S G, Shaldzhian, A A, Nalbandian, R M, Buniatian, G Kh
Format:	Journal Article
Language:	Russian
Published:	Armenia (Republic) 1975
Subjects:	Animals Cattle Cerebral Cortex - analysis Chemical Phenomena Chemistry Cytochrome c Group - isolation & purification Electrophoresis, Polyacrylamide Gel Iron Isoelectric Point Molecular Weight Oxidation-Reduction Spectrum Analysis
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Summary:	Cytochrome c from grey matter of brain has been obtained as a homogeneous preparation by electrophoresis on polyacrylamide gel and following electrofocusing in ampholine solutions. Its molecular weight, content of iron, redox potential and isoelectric point have been established. The absolute spectra of its oxidized and reduced forms are presented. Cytochrome c of brain cortex is similar in its properties to that obtained from other animal tissues as the heart and adrenal cortex.
ISSN:	0507-2972