Possible role of neuropeptides in regulating nucleic acid biosynthesis in the brain

• Published in: Voprosy biokhimii mozga Vol. 13; p. 206
• Main Author: Khachatrian, G S
• Format: Journal Article
• Language: Russian
• Published: Armenia (Republic) 1978
• Subjects: Amanitins - pharmacology; Amino Acid Sequence; Animals; Brain - metabolism; Brain Chemistry - drug effects; Cell Nucleus - analysis; Chemical Phenomena; Chemistry; Cyclic AMP - physiology; Cyclic GMP - physiology; DNA-Directed RNA Polymerases - antagonists & inhibitors; DNA-Directed RNA Polymerases - isolation & purification; DNA-Directed RNA Polymerases - metabolism; Lypressin - analogs & derivatives; Lypressin - pharmacology; Protein Kinases - metabolism; Rats; RNA - biosynthesis; RNA, Messenger - biosynthesis; RNA, Ribosomal - analysis; RNA, Transfer - analysis; Time Factors; Transcription, Genetic - drug effects
• ISSN: 0507-2972

It has been studied the role of intracysternal administration of neuropeptides--five analogous of 8-lysinvasopressin (LVP) [Des-9-Gly-cine-amide-LVP (DGLVP), Tri-Gly-LVP, deamino-LVP (1--beta-mercaptopropionic acid-LVP), Leu4-LVP, Asn1,6-LVP] and two cardiotrop hexapeptides (acetylated and non) separated from the hypothalamus in the regulation of various types of RNA (GC and AU types n-RNA, r-RNA, t-RNA) biosynthesis in the brain. Using the combined method of phenol extraction, differential ultracentrifugation, gel-filtration and ultraviolet spectroscory of various types of RNA has been shown the increase in the content of r-RNA and t-RNA under the influence of DGLVP, Leu4-LVP and cardiotrop hexapeptide. Deamino-LVP, Tri-Gly-LVP and acetylated hexapeptide stimulate the rise in the content of r-RNA and AU type n-RNA (precursor of m-RNA). Ans1,6-LVP shows a nonspecific influence on the biosynthesis of the brain RNA. It has been suggested a hypothesis about a participation of the neuropeptides in the regulation of various types of the brain RNA biosynthesis, as a primary messanger (starting signals) during the stimulation of the initiation of cycloadenylate- and cycloguanylate-sensitive transcription, via phosphorylation of diverse RNA-polymerases. It was given a scheme of interaction between the messanger conception of neuropeptides and cyclic-3', 5'-monophosphate nucleotide-dependent transcription by which the cycloadenylate-dependent transcription appears to play a great role in the r-RNA and t-RNA biosynthesis and the cycloguanylate-dependent transcription -- in the biosynthesis of m-RNA.