Comparative studies on the detection of Y-chromatin in native and formalin-fixed tissue or organ material (author's transl)

Comparative studies on the detection of Y-chromatin in native and formalin-fixed tissue or organ material (author's transl)

Frequency of detectable Y-bodies in interphase nuclei after mepacrin staining of paraffin embedded formalin-fixed tissue was compared with those of cryostat sections of the native material and organ smears with and without formalin fixing. In formalin fixed smears from males 42--70% of investigated...

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Bibliographic Details
Published in:Zentralblatt fur allgemeine Pathologie u. pathologische Anatomie Vol. 120; no. 1; p. 61
Main Authors: Schmechta, H, Porstmann, T
Format: Journal Article
Language:German
Published: Germany 1976
Subjects:
Brain - cytology
Fluorescence
Histological Techniques
Humans
Kidney - cytology
Liver - cytology
Male
Sex Chromatin - analysis
Spleen - cytology
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Summary:Frequency of detectable Y-bodies in interphase nuclei after mepacrin staining of paraffin embedded formalin-fixed tissue was compared with those of cryostat sections of the native material and organ smears with and without formalin fixing. In formalin fixed smears from males 42--70% of investigated cells showed the Y-body. Sections of formalin-fixed and paraffin embedded male organs demonstrated in 12--24% of their cells the typical fluorescence for the Y-chromatin. High significantly less fluorescent Y-like bodies were found in smears and sections of all proofed organs from women prepared in the same manner. Mepacrin staining in combination with investigation of Barr-bodies permits a fast determination of sex of both organ smears and sections, too.
ISSN:0044-4030