Immunochemical study of the specific antigens of human amniotic fluid

• Published in: Ontogenez Vol. 10; no. 4; p. 389
• Main Authors: Kalashnikov, V V, Vasil'ev, M Iu, Voloshchuk, S G, Falaleeva, D M, Rykunov, E I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 1979
• Subjects: Alpha-Globulins - analysis; Amniotic Fluid - immunology; Animals; Antigens - analysis; Beta-Globulins - analysis; Epitopes; Female; Gestational Age; Humans; Immune Sera - isolation & purification; Immunization; Immunodiffusion - methods; Immunoelectrophoresis - methods; Immunoelectrophoresis, Two-Dimensional - methods; Infant, Newborn; Prealbumin - analysis; Pregnancy; Rabbits; Time Factors
• ISSN: 0475-1450

Four specific antigens (trophoblastic beta 1-globulin, placental lactogen, alpha 1- and alpha 2-globulins of human placenta) were identified using antisera to the native amniotic fluid. Five antigens with the mobility of prealbumins, alpha 1-globulins, alpha 2-globulins and beta 2-globulins which bear no resemblance with the previously studied antigens were identified using antisera to the acid fraction of the amniotic fluid. Both the prealbumins and alpha 2-globulin were found in the blood serum of foetuses of different age and of newborn infants; these proteins were absent from the blood serum of pregnant women and donors. They received the names of embryonic prealbumine 1, embryonic prealbumine 2 and embryonic alpha 2-globulin. The protein with the mobility of alpha 1-globulins was found in the amniotic fluid of foetuses and in the blood serum of pregnant women only and received the name of amniotic alpha 1-globulin. The concentration of the antigens in question was studied in the developing foetuses and in the blood serum of pregnant women at different stages of pregnancy.