Improved detection of Mycobacterium bovis in bovine tissues using immunomagnetic separation approaches

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1247; p. 153
• Main Authors: Grant, Irene R, Stewart, Linda D
• Format: Journal Article
• Language: English
• Published: United States 01-01-2015
• Subjects: Animals; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - microbiology; Immunomagnetic Separation - methods; Molecular Diagnostic Techniques - methods; Molecular Diagnostic Techniques - standards; Mycobacterium bovis - genetics; Mycobacterium bovis - isolation & purification; Tuberculosis - veterinary
• ISSN: 1940-6029
• DOI: 10.1007/978-1-4939-2004-4_11

Immunomagnetic separation (IMS) represents a simple but effective method of selectively capturing and concentrating Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), from tissue samples. It is a physical cell separation technique that does not impact cell viability, unlike traditional chemical decontamination prior to culture. IMS is performed with paramagnetic beads coated with M. bovis-specific antibody and peptide binders. Once captured by IMS, M. bovis cells can be detected by either PCR or cultural detection methods. Increased detection rates of M. bovis, particularly from non-visibly lesioned lymph node tissues from bTB reactor animals, have recently been reported when IMS-based methods were employed.