Sequences homologous to the human D1S1 locus present on human chromosome 3

• Published in: American journal of human genetics Vol. 38; no. 4; pp. 428 - 436
• Main Authors: CARRITT, B, WELCH, H. M, PARRY-JONES, N. J
• Format: Journal Article
• Language: English
• Published: Chicago, IL University of Chicago Press 01-04-1986
• Subjects: Animals; Bacteriophage lambda - genetics; Base Sequence; Biological and medical sciences; Chromosome Mapping; Chromosomes, Human, 1-3; Cloning, Molecular; Cricetinae; Cricetulus; DNA Restriction Enzymes; Fundamental and applied biological sciences. Psychology; Genes. Genome; Genetic Linkage; Genetic Markers; Humans; Hybrid Cells; Mice; Molecular and cellular biology; Molecular genetics; Nucleic Acid Hybridization; Polymorphism, Genetic; Rabbits; Rats; Human; Molecular hybridization; Hybrid cell; Gene; Repeated sequence; DNA; In situ; Somatic cell; Homology; Molecular cloning; A1-Chromosome; A3»-Chromosome
• ISSN: 0002-9297; 1537-6605

We have investigated the segregation, in somatic cell hybrids, of the human D1S1 locus, previously assigned to 1p36 by in situ hybridization. We have shown that the clone which defines this locus, lambda Ch4A-H3, originates from human chromosome 3, but contains a 1.7-kilobase (kb) PstI-HindIII repetitive element that is also present on chromosome 1, probably distal to PGD. The clone recognizes restriction fragment length polymorphisms within the single-copy sequence on chromosome 3 and one for the enzyme StuI in the repeated sequence on chromosome 1. These experiments thus expose a level of complexity in the D1S1 locus not revealed by earlier in situ hybridization studies.